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Background  Ecological field research on the influence of meteorological parameters on a forest inhabiting species is confronted with the complex relations between measured data and the real conditions the species is exposed to. This study highlights this complexity for the example of  Ixodes ricinus . This species lives mainly in forest habitats near the ground, but field research on impacts of meteorological conditions on population dynamics is often based on data from nearby official weather stations or occasional in situ measurements. In addition, studies use very different data approaches to analyze comparable research questions. This study is an extensive examination of the methodology used to analyze the impact of meteorological parameters on  Ixodes ricinus  and proposes a methodological approach that tackles the underlying complexity.    Methods  Our specifically developed measurement concept was implemented at 25 forest study sites across Baden-Württemberg, Germany. Meteorological weather stations recorded data in situ and continuously between summer 2012 and autumn 2015, including relative humidity measures in the litter layer and different heights above it (50 cm, 2 m). Hourly averages of relative humidity were calculated and compared with data from the nearest official weather station.    Results  Data measured directly in the forest can differ dramatically from conditions recorded at official weather stations. In general, data indicate a remarkable relative humidity decrease from inside to outside the forest and from ground to atmosphere. Relative humidity measured in the litter layer were, on average, 24% higher than the official data and were much more balanced, especially in summer.    Conclusions  The results illustrate the need for, and benefit of, continuous in situ measurements to grasp the complex relative humidity conditions in forests. Data from official weather stations do not accurately represent actual humidity conditions in forest stands and the explanatory power of short period and fragmentary in situ measurements is extremely limited. However, it is still an open question to what kind of meteorological data are necessary to answer specific questions in tick research. The comparison of research findings was hindered by the variety of information provided, which is why we propose details for future reporting.

On the complexity of measuring forests microclimate and interpreting its relevance in habitat ecology: the example of Ixodes ricinus ticks