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Background  With the increasing interest in vaccines to interrupt malaria transmission, there is a demand for harmonization of current methods to assess  Plasmodium  transmission in laboratory settings. Potential vaccine candidates are currently tested in the standard membrane feeding assay (SMFA) that commonly relies on  Anopheles stephensi  mosquitoes. Other mosquito species including  Anopheles gambiae  are the dominant malaria vectors for  Plasmodium falciparum  in sub-Saharan Africa.    Methods  Using human serum and monoclonal pre-fertilization (anti-Pfs48/45) and post-fertilization (anti-Pfs25) antibodies known to effectively inhibit sporogony, we directly compared SMFA based estimates of transmission-reducing activity (TRA) for  An. stephensi  and  An. gambiae  mosquitoes.    Results  In the absence of transmission-reducing antibodies, average numbers of oocysts were similar between  An. gambiae  and  An. stephensi . Antibody-mediated TRA was strongly correlated between both mosquito species, and absolute TRA estimates for pre-fertilisation monoclonal antibodies (mAb) showed no significant difference between the two species. TRA estimates for IgG of naturally exposed individuals and partially effective concentrations of anti-Pfs25 mAb were higher for  An. stephensi  than for  An. gambiae .    Conclusion  Our findings support the use of  An. stephensi  in the SMFA for target prioritization. As a vaccine moves through product development, better estimates of TRA and transmission-blocking activity (TBA) may need to be obtained in epidemiologically relevant parasite-species combination.    Electronic supplementary material  The online version of this article (10.1186/s13071-017-2414-z) contains supplementary material, which is available to authorized users.

parasites and vectors

Comparative assessment of An. gambiae and An. stephensi mosquitoes to determine transmission-reducing activity of antibodies against P. falciparum sexual stage antigens