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Background  Glutathione-S-transferase (GST) is a widespread multigene family of detoxification enzymes. The vaccination of mice with recombinant GST of 24 kDa from  Trichinella spiralis  elicited a low immune protection against challenge infection. The objective of this study was to characterize the  T. spiralis  putative GST gene ( TspGST ) encoding a 30.8 kDa protein and to evaluate its potential as a candidate antigen for anti- Trichinella  vaccine.    Methods  The full-length cDNA sequence of  TspGST  from  T. spiralis  muscle larvae (ML) was expressed in  E. coli . The enzymatic activity and antigenicity of the rTspGST were identified by spectrophotometry, Western blot, and ELISA. The expression of  TspGST  at  T. spiralis  various stages was investigated by RT-PCR and indirect immunofluorescent test (IIFT). Serum level of total IgG, IgG1, and IgG2a antibodies against rTspGST were measured by ELISA. The immune protection produced by vaccination with rTspGST against  T. spiralis  was evaluated.    Results  The sequencing results showed that the cDNA of  TspGST  was 840 bp, and encoded a protein of 279 amino acids, which had a molecular size of 30.8 kDa and a pI of 5.21. Its amino acid sequence shares 37% similarity with TsGST. The rTspGST protein had enzymatic activity of GST. On Western blot and ELISA analysis, the native TspGST protein with 30.8 kDa in crude antigens derived from adult worms (AW), newborn larvae (NBL), infective intestinal larvae (IIL) and ML was recognized by anti-rTspGST sera, but the ML ES antigens could be not recognized by anti-rTspGST sera. Expression of  TspGST  was found in all of  T. spiralis  various stages (AW, NBL, ML, and IIL). An immunolocalization analysis identified TspGST in different stages (mainly in cuticles) of the nematode. The mice vaccinated with the rTspGST elicited Th2-predominant immune responses, showed a 34.38% reduction of adult worms and a 43.70% reduction of muscle larvae.    Conclusions  Immunization with rTspGST produced a partial immune protection, and the rTspGST could be regarded as a potential candidate target for an anti- Trichinella  vaccine.

Cloning and expression of a Trichinella spiralis putative glutathione S-transferase and its elicited protective immunity against challenge infections