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Background  Ticks are among the most harmful vectors worldwide. Their salivary glands play essential roles in blood-feeding and pathogen transmission and undergo apoptosis after feeding. Although it was previously reported that salivary degeneration in ixodid ticks is in response to hormonal stimulation, questions still exist with the underlying mechanisms of salivary gland apoptosis.    Methods  Salivary glands of  Rhipicephalus haemaphysaloides  were collected from 1 to 7 days after attachment to the host. TUNEL and Annexin V assays were used to check apoptosis during this time. To confirm the role of caspase-1, RNA interference was used to silence its expression, and the dynamic changes of associated cysteine proteases were also shown by quantitative real time PCR and western blot, while TUNEL and Annexin V assays were used to confirm apoptosis.    Results  In the present study, apoptosis of salivary glands in  R. haemaphysaloides  occurred 3 or 4 days after attachment to the host as determined by TUNEL and Annexin V assays. The expression of caspase-1 increased at 5–7 days. When the latter was silenced by RNA interference, apoptosis in the salivary glands was delayed. While there seemed to be another form of cell death in salivary glands of ticks, such occurrence may be caused by compensatory autophagy which involved autophagy-related gene 4D.    Conclusions  This study describes the apoptosis of salivary glands in  R. haemaphysaloides  and the dynamic changes in cysteine proteases in this activity. Cysteine proteases were involved in this process, especially caspase-1. Caspase-1 participated in the apoptosis of salivary glands.    Electronic supplementary material  The online version of this article (doi:10.1186/s13071-017-2161-1) contains supplementary material, which is available to authorized users.

Caspase-1 participates in apoptosis of salivary glands in Rhipicephalus haemaphysaloides