English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background    Giardia duodenalis  is an important protozoan parasite. It is an established zoonotic pathogen and dairy calves have been implicated as one of the most important sources of human infection. This study was conducted to assess the prevalence and multilocus genotyping of  G. duodenalis  in dairy calves in the Xinjiang Uyghur Autonomous Region, northwestern China.    Findings  A total of 514 fresh fecal samples were randomly collected from dairy calves in 15 farms in Xinjiang, 13.4 % (69/514) tested positive for  G. duodenalis  by polymerase chain reaction (PCR) detection of the small subunit ribosomal RNA (SSU rRNA) gene, with the prevalence being 9.7 % (23/237) and 16.6 % (46/277) in pre- and post-weaned calves, respectively. Sequence analysis of the SSU rRNA gene predominantly detected  G. duodenalis  assemblage E (92.8 %, 64/69), whereas assemblage A was identified in five samples (7.2 %, 5/69). All  G. duodenalis -positive samples were assayed with PCR followed by sequencing at β-giardin ( bg ), glutamate dehydrogenase ( gdh ) and triosephosphate isomerase ( tpi ) genes, and 29, 37 and 33 sequences were obtained, respectively. The presence of mixed  G. duodenalis  assemblage A and E was detected in only one sample. Multilocus genotyping yielded 15 multilocus genotypes (MLGs), one new assemblage A MLG, and 14 assemblage E MLGs. All assemblage E MLGs identified here differed genetically from those of cattle from Henan Province, Central China.    Conclusions  Our data indicate that  G. duodenalis  is a common parasite in dairy calves in Xinjiang, China, and calves appear to be a reservoir of  G. duodenalis  that is infectious to humans. The differences in the distribution of  G. duodenalis  assemblage E MLGs from cattle were likely to be because of geographical segregation.

Prevalence and multilocus genotyping of Giardia duodenalis in dairy calves in Xinjiang, Northwestern China