English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background   Acanthamoeba  spp. are free-living ubiquitous protozoans capable of causing  Acanthamoeba  meningitis/meningoencephalitis (AME) of the central nervous system in humans.  Acanthamoeba  spp. are divided into 20 different genotypes (T1–T20) on the basis of variation in nucleotide sequences of the 18S rRNA gene. The objective of this study was to identify the genotypes of  Acanthamoeba  spp. in patients of  Acanthamoeba  meningitis/meningoencephalitis (AME) using 18S rRNA gene-based PCR assay. The present study provides information regarding the involvement of the most prevalent and predominant genotype of  Acanthamoeba  spp. in  Acanthamoeba  meningitis/meningoencephalitis infections in India.    Methods  Cerebrospinal fluid (CSF) was collected from 149 clinically suspected  Acanthamoeba  meningitis/meningoencephalitis (AME) patients reporting to the outpatient department/causality services of the Neurosciences Centre, AIIMS, New Delhi, India during the past five years. Samples were inoculated onto 2 % non-nutrient agar plates overlaid with  E. coli  and incubated at 30 °C for 14 days. Among 149 suspected patients, ten were found culture-positive for  Acanthamoeba  spp. out of which six isolates were established in axenic culture for molecular analysis. DNA was isolated and a PCR assay was performed for amplification of the Diagnostic fragment 3 (DF3) (~280 bp) region of the 18S rRNA gene from axenic culture of six  Acanthamoeba  spp. isolates . Rns  genotyping was performed on the basis of the variation in nucleotide sequences of DF3 region of the 18S rRNA gene.    Results  In the phylogenetic analysis, all of the six  Acanthamoeba  spp. isolates were found to belong to genotype T4. The sequence homology search for these six isolates in the NCBI databank showed homology with the available strains of  Acanthamoeba  spp .  The newly generated sequences are available in the GenBank database under accession numbers KT004416–KT004421.    Conclusions  In the present study, genotype T4 was found as the most prevalent and predominant genotype in  Acanthamoeba  meningitis/ meningoencephalitis infections. Hence further studies are needed to develop optimal therapeutic strategy against  Acanthamoeba  spp. of genotype T4 to combat against the infections.

Isolation and genotyping of Acanthamoeba spp. from Acanthamoeba meningitis/ meningoencephalitis (AME) patients in India