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Background  The cattle tick  Rhipicephalus  ( Boophilus )  microplus  is an economically important parasite of livestock. Effective control of ticks using acaricides is threatened by the emergence of resistance to many existing compounds. Several continuous  R. microplus  cell lines have been established and provide an under-utilised resource for studies into acaricide targets and potential genetic mutations associated with resistance. As a first step to genetic studies using these resources, this study aimed to determine the presence or absence of two genes and their transcripts that have been linked with acaricide function in cattle ticks: β-adrenergic octopamine receptor ( βAOR , associated with amitraz resistance) and ATP-binding cassette B10 ( ABCB10 , associated with macrocyclic lactone resistance) in six  R. microplus  cell lines, five other  Rhipicephalus  spp. cell lines and three cell lines representing other tick genera ( Amblyomma variegatum ,  Ixodes ricinus  and  Hyalomma anatolicum ).    Methods  End-point polymerase chain reaction (PCR) was used for detection of the  βAOR  gene and transcripts in DNA and RNA extracted from the tick cell lines, followed by capillary sequencing of amplicons. Quantitative real-time PCR (qPCR) was performed to determine the levels of expression of  ABCB10 .    Results   βAOR  gene expression was detected in all  Rhipicephalus  spp. cell lines. We observed a second amplicon of approximately 220 bp for the  βAOR  gene in the  R. microplus  cell line BME/CTVM6, derived from acaricide-resistant ticks. Sequencing of this transcript variant identified a 36 bp insertion in the  βAOR  gene, leading to a 12-amino acid insertion (LLKTLALVTIIS) in the first transmembrane domain of the protein. In addition, nine synonymous SNPs were also discovered in  R. appendiculatus ,  R. evertsi  and  R. sanguineus  cell lines. Some of these SNPs appear to be unique to each species, providing potential tools for differentiating the tick species. The BME/CTVM6 cell line had significantly higher  ABCB10  ( P  = 0.002) expression than the other  R. microplus  cell lines.    Conclusions  The present study has identified a new  βAOR  gene and demonstrated a higher  ABCB10  expression level in the BME/CTVM6 cell line, indicating that tick cell lines provide a useful experimental tool for acaricide resistance studies and further elucidation of tick genetics.    Electronic supplementary material  The online version of this article (doi:10.1186/s13071-016-1708-x) contains supplementary material, which is available to authorized users.

parasites and vectors

Identification of a novel β-adrenergic octopamine receptor-like gene (βAOR-like) and increased ATP-binding cassette B10 (ABCB10) expression in a Rhipicephalus microplus cell line derived from acaricide-resistant ticks