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Background  Quantification of eggs prior to rearing the immature stages of mosquitoes is an essential step in establishing a standardized mass rearing system. To develop a simple and accurate method of egg quantification for  Aedes aegypti  and  Aedes albopictus , the relationship between egg number and weight, as well as egg number and volume, were studied.    Methods  Known quantities of eggs (1,000, 3,000, 6,000, 12,000, 15,000, 18,000, 21,000 and 27,000) were counted and subsequently their weight and volume were measured. Best-fit curves and regression equations were used to describe relationships between  Aedes  egg number and both weight and volume.    Results  Eighteen thousand  Ae. aegypti  eggs weighed 159.8 mg and had a volume of 277.4 μl, compared to measurements of 131.5 mg and 230.3 μl for  Ae. albopictus . The eggs of  Ae. aegypti  were thus larger and heavier than those of  Ae. albopictus . The use of weight and volume to quantify egg number was validated by counting volumes and weights of eggs expected to correspond to 3,000 and 18,000 eggs of each species; significant correlations were found in all cases except in the case of 3,000  Ae. albopictus  eggs measured by volume.    Conclusion  Methods for egg quantification were validated and shown to be a consistent and practical means to achieve uniform distribution of  Aedes  larvae between rearing trays, important for optimal mass rearing of the immature stages of  Aedes  mosquitoes.

parasites and vectors

Standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors Aedes aegypti and Aedes albopictus (Diptera: Culicidae) - I - egg quantification