English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Biomass valorization has been suggested as a sustainable alternative to petroleum-based energy and commodities. In this context, the copper radical oxidases (CROs) from Auxiliary Activity Family 5/Subfamily 2 (AA5_2) are attractive biocatalysts for the selective oxidation of primary alcohols to aldehydes. Originally defined by the archetypal galactose 6-oxidase from  Fusarium graminearum , fungal AA5_2 members have recently been shown to comprise a wide range of specificities for aromatic, aliphatic and furan-based alcohols. This suggests a broader substrate scope of native CROs for applications. However, only 10% of the annotated AA5_2 members have been characterized to date.    Results  Here, we define two homologues from the filamentous fungi  Fusarium graminearum  and  F. oxysporum  as predominant aryl alcohol oxidases (AAOs) through recombinant production in  Pichia pastoris , detailed kinetic characterization, and enzyme product analysis. Despite possessing generally similar active-site architectures to the archetypal  Fgr GalOx,  Fgr AAO and  Fox AAO have weak activity on carbohydrates, but instead efficiently oxidize specific aryl alcohols. Notably, both  Fgr AAO and  Fox AAO oxidize hydroxymethyl furfural (HMF) directly to 5-formyl-2-furoic acid (FFCA), and desymmetrize the bioproduct glycerol to the uncommon L-isomer of glyceraldehyde.    Conclusions  This work expands understanding of the catalytic diversity of CRO from AA5_2 to include unique representatives from  Fusarium  species that depart from the well-known galactose 6-oxidase activity of this family. Detailed enzymological analysis highlights the potential biotechnological applications of these orthologs in the production of renewable plastic polymer precursors and other chemicals.    Supplementary Information  The online version contains supplementary material available at 10.1186/s13068-021-01984-0.

Two Fusarium copper radical oxidases with high activity on aryl alcohols