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Background  The biomanufacturing of  d -glucaric acid has attracted increasing interest because it is one of the top value-added chemicals produced from biomass.  Saccharomyces cerevisiae  is regarded as an excellent host for  d- glucaric acid production.    Results  The  opi1  gene was knocked out because of its negative regulation on  myo -inositol synthesis, which is the limiting step of  d -glucaric acid production by  S. cerevisiae . We then constructed the biosynthesis pathway of  d -glucaric acid in  S. cerevisiae  INVSc1  opi1Δ  and obtained two engineered strains, LGA-1 and LGA-C, producing record-breaking titers of  d -glucaric acid: 9.53 ± 0.46 g/L and 11.21 ± 0.63 g/L  d -glucaric acid from 30 g/L glucose and 10.8 g/L  myo -inositol in fed-batch fermentation mode, respectively. However, LGA-1 was preferable because of its genetic stability and its superior performance in practical applications. There have been no reports on  d -glucaric acid production from lignocellulose. Therefore, the biorefinery processes, including separated hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP) were investigated and compared. CBP using an artificial microbial consortium composed of  Trichoderma reesei  ( T. reesei ) Rut-C30 and  S. cerevisiae  LGA-1 was found to have relatively high  d -glucaric acid titers and yields after 7 d of fermentation, 0.54 ± 0.12 g/L  d -glucaric acid from 15 g/L Avicel and 0.45 ± 0.06 g/L  d -glucaric acid from 15 g/L steam-exploded corn stover (SECS), respectively. In an attempt to design the microbial consortium for more efficient CBP, the team consisting of  T. reesei  Rut-C30 and  S. cerevisiae  LGA-1 was found to be the best, with excellent work distribution and collaboration.    Conclusions  Two engineered  S. cerevisiae  strains, LGA-1 and LGA-C, with high titers of  d -glucaric acid were obtained. This indicated that  S. cerevisiae  INVSc1 is an excellent host for  d -glucaric acid production. Lignocellulose is a preferable substrate over  myo -inositol. SHF, SSF, and CBP were studied, and CBP using an artificial microbial consortium of  T. reesei  Rut-C30 and  S. cerevisiae  LGA-1 was found to be promising because of its relatively high titer and yield.  T. reesei  Rut-C30 and  S. cerevisiae  LGA-1were proven to be the best teammates for CBP. Further work should be done to improve the efficiency of this microbial consortium for  d -glucaric acid production from lignocellulose.    Supplementary Information  The online version contains supplementary material available at 10.1186/s13068-021-01961-7.

Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium