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Background  Predatory flagellates and ciliates are two common bio-contaminants which frequently cause biomass losses in  Chlorella  mass culture. Efficient and targeted ways are required to control these contaminations in  Chlorella  mass cultivation aiming for biofuel production especially.    Results  Five surfactants were tested for its ability to control bio-contaminations in  Chlorella  culture. All five surfactants were able to eliminate the contaminants at a proper concentration. Particularly the minimal effective concentrations of sodium dodecyl benzene sulfonate (SDBS) to completely eliminate  Poterioochromonas  sp. and  Hemiurosomoida  sp. were 8 and 10 mg L −1 , respectively, yet the photosynthesis and viability of  Chlorella  was not significantly affected. These results were further validated in  Chlorella  mass cultures in 5, 20, and 200 m 2  raceway ponds.    Conclusions  A chemical method using 10 mg L −1  SDBS as pesticide to control predatory flagellate or ciliate contamination in  Chlorella  mass culture was proposed. The method helps for a sustained microalgae biomass production and utilization, especially for biofuel production.

Controlling of two destructive zooplanktonic predators in Chlorella mass culture with surfactants