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Background  Capture and storage of the energy carrier hydrogen as well as of the greenhouse gas carbon dioxide are two major problems that mankind faces currently. Chemical catalysts have been developed, but only recently a group of anaerobic bacteria that convert hydrogen and carbon dioxide to acetate, formate, or biofuels such as ethanol has come into focus, the acetogenic bacteria. These biocatalysts produce the liquid organic hydrogen carrier formic acid from H 2  + CO 2  or even carbon monoxide with highest rates ever reported. The autotrophic, hydrogen-oxidizing, and CO 2 -reducing acetogens have in common a specialized metabolism to catalyze CO 2  reduction, the Wood–Ljungdahl pathway (WLP). The WLP does not yield net ATP, but is hooked up to a membrane-bound respiratory chain that enables ATP synthesis coupled to CO 2  fixation. The nature of the respiratory enzyme has been an enigma since the discovery of these bacteria and has been unraveled in this study.    Results  We have produced a His-tagged variant of the ferredoxin:NAD oxidoreductase (Rnf complex) from the model acetogen  Acetobacterium woodii , solubilized the enzyme from the cytoplasmic membrane, and purified it by Ni 2+ –NTA affinity chromatography. The enzyme was incorporated into artificial liposomes and catalyzed Na +  transport coupled to ferredoxin-dependent NAD reduction. Our results using the purified enzyme do not only verify that the Rnf complex from  A. woodii  is Na + -dependent, they also demonstrate for the first time that this membrane-embedded molecular engine creates a Na +  gradient across the membrane of  A. woodii  which can be used for ATP synthesis.    Discussion  We present a protocol for homologous production and purification for an Rnf complex. The enzyme catalyzed electron-transfer driven Na +  export and, thus, our studies provided the long-awaited biochemical proof that the Rnf complex is a respiratory enzyme.

biotechnology for biofuels

Homologous production, one-step purification, and proof of Na+ transport by the Rnf complex from Acetobacterium woodii, a model for acetogenic conversion of C1 substrates to biofuels