English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Switchgrass ( Panicum virgatum ) is a warm-season perennial grass. Improving its cold tolerance is important for its sustainable production in cooler regions. Through genome-wide bioinformatic analysis of switchgrass  Zinc finger - CCCH  genes ( PvC3Hs ), we found that several  PvC3Hs , including  PvC3H72 , might play regulatory roles in plant cold tolerance. The objectives of this study were to characterize  PvC3H72  using reverse genetics approach and to understand its functional role in cold signal transduction and cold tolerance in switchgrass.    Results   PvC3H72  is an intronless gene encoding a transcriptional activation factor. The expression of  PvC3H72  was rapidly and highly induced by cold stress. Transgenic switchgrass with over-expressed  PvC3H72  driven under maize ubiquitin promoter showed significantly improved chilling tolerance at 4 °C as demonstrated by less electrolyte leakage and higher relative water content than wild-type (WT) plants, as well as significantly higher survival rate after freezing treatment at − 5 °C. Improved cold tolerance of  PvC3H72  transgenic lines was associated with significantly up-regulated expression of  ICE1 – CBF – COR  regulon and ABA-responsive genes during cold treatment.    Conclusions   PvC3H72  was the first characterized switchgrass cold-tolerance gene and also the only  Znf - CCCH  family gene known as a transcription factor in plant cold tolerance. PvC3H72 was an added signaling component in plant cold tolerance associated with regulation of ICE1–CBF–COR regulon and ABA-responsive genes. Knowledge gained in this study not only added another acting component into plant cold-tolerance mechanism, but also be of high value for genetic improvement of cold tolerance in switchgrass as well as other warm-season grasses.

biotechnology for biofuels

Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes