English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  High temperature inhibits cell growth and ethanol fermentation of  Saccharomyces cerevisiae . As a complex phenotype, thermotolerance usually involves synergistic actions of many genes, thereby being difficult to engineer. The overexpression of either endogenous or exogenous stress-related transcription factor genes in yeasts was found to be able to improve relevant stress tolerance of the hosts.    Results  To increase ethanol yield of high-temperature fermentation, we constructed a series of strains of  S. cerevisiae  by expressing 8 transcription factor genes from  S. cerevisiae  and 7 transcription factor genes from thermotolerant  K. marxianus  in  S. cerevisiae . The results of growth curve measurements and spotting test show that  Km Hsf1 and  Km Msn2 can enhance cell growth of  S. cerevisiae  at 40–42 °C. According to the results of batch fermentation at 43 °C with an initial glucose concentration of 104.8 g/l, the fermentation broths of  KmHSF1  and  KmMSN2 -expressing strains could reach final ethanol concentrations of 27.2 ± 1.4 and 27.6 ± 1.2 g/l, respectively, while the control strain just produced 18.9 ± 0.3 g/l ethanol. Transcriptomic analysis found that the expression of  KmHSF1  and  KmMSN2  resulted in 55 (including 31 up-regulated and 24 down-regulated) and 50 (including 32 up-regulated and 18 down-regulated) genes with different expression levels, respectively (padj < 0.05). The results of transcriptomic analysis also reveal that  Km Hsf1 might increase ethanol production by regulating genes related to transporter activity to limit excessive ATP consumption and promote the uptake of glucose; while  Km Msn2 might promote ethanol fermentation by regulating genes associated with glucose metabolic process and glycolysis/gluconeogenesis. In addition,  Km Msn2 might also help to cope with high temperature by regulating genes associated with lipid metabolism to change the membrane fluidity.    Conclusions  The transcription factors  Km Hsf1 and  Km Msn2 of thermotolerant  K. marxianus  can promote both cell growth and ethanol fermentation of  S. cerevisiae  at high temperatures. Different mechanisms of  Km Hsf1 and  Km Msn2 in promoting high-temperature ethanol fermentation of  S. cerevisiae  were revealed by transcriptomic analysis.    Electronic supplementary material  The online version of this article (10.1186/s13068-017-0984-9) contains supplementary material, which is available to authorized users.

biotechnology for biofuels

The transcription factors Hsf1 and Msn2 of thermotolerant Kluyveromyces marxianus promote cell growth and ethanol fermentation of Saccharomyces cerevisiae at high temperatures