English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated primarily for lipid production. However, yeasts store neutral lipids intracellularly making recovery difficult and expensive. In addition, once recovered from the cells, lipids are difficult to blend directly with the existing fuels without upgrading. We have, therefore, begun to investigate secreted fatty acid-derived products which can be easily recovered and upgraded to fuels.    Results  In this study, we successfully demonstrate the production of fatty alcohols by the oleaginous yeasts,  Yarrowia lipolytica  and  Lipomyces starkeyi , through expression of the fatty acyl-CoA reductase gene from  Marinobactor aquaeolei  VT8. This strategy resulted in the production of 167 and 770 mg/L of fatty alcohols in shake flask from  Y. lipolytica  and  L starkeyi , respectively. When using a dodecane overlay during fermentation, 92 and 99% of total fatty alcohols produced by  Y. lipolytica  and  L. starkeyi , respectively, were extracted into the dodecane phase, which compares favorably to the 3 and 50% recovered, respectively, without the dodecane layer. In both oleaginous yeasts, long chain length, saturated fatty alcohols, i.e., hexadecanol (C16:0) and octadecanol (C18:0), were predominant and accounted for more than 85% of the total fatty alcohols produced. To the best of our knowledge, this is the first report of fatty alcohol production in  L. starkeyi.Conclusion  This work demonstrates that the oleaginous yeasts,  Y. lipolytica  and  L. starkeyi , can serve as platform organisms for the production of fatty acid-derived biofuels and bioproducts.

Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica