English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  Microalgae biofuel has become the most promising renewable energy over the past few years. But limitations still exist because of its high cost. Although, efforts have been made in enhancement of lipid productivity, the major cost problem in harvesting and oil extraction is still intractable. Thus, the idea of fatty acids (FAs) secretion which can massively facilitate algae harvesting and oil extraction was investigated here.    Results  The cDNAs of two long-chain acyl-CoA synthetases (LACSs) genes were cloned from  Chlamydomonas reinhardtii  and named as  cracs1  and  cracs2 . They showed different substrate adaptation in the yeast complementation experiments.  Cracs2  could utilize FAs C12:0, C14:0, C16:0, C18:0, C16:1 and C18:1, while  crac1  could only utilize substrate C14:0, C16:1 and C18:1. Knockdown of  cracs1  and  cracs2  in  C. reinhardtii  resulted in accumulation of intracellular lipids. The total intracellular lipids contents of transgenic algae q-15 (knockdown of  cracs1 ) and p-13 (knockdown of  cracs2 ) were 45 and 55 %, respectively higher than that of cc849. Furthermore, FAs secretion was discovered in both transgenic algae. Secreted FAs can reach 8.19 and 9.66 mg/10 9  cells in q-15 and p-13, respectively.    Conclusion  These results demonstrated the possibility of FAs secretion by microalgae and may give a new strategy of low-cost oil extraction. According to our findings, we proposed that FAs secretion may also be achieved in other species besides  Chlamydomonas reinhardtii  by knocking-down  cracs  genes, which may promote the future industrial application of microalgae biofuels.    Electronic supplementary material  The online version of this article (doi:10.1186/s13068-016-0598-7) contains supplementary material, which is available to authorized users.

Characterization of long-chain acyl-CoA synthetases which stimulate secretion of fatty acids in green algae Chlamydomonas reinhardtii