English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The ability to deconstruct plant biomass without conventional pretreatment has made members of the genus  Caldicellulosiruptor  the target of investigation for the consolidated processing of lignocellulosic biomass to biofuels and bioproducts. These Gram-positive bacteria are hyperthermophilic anaerobes and the most thermophilic cellulolytic organisms so far described. They use both C5 and C6 sugars simultaneously and have the ability to grow well on xylan, a major component of plant cell walls. This is an important advantage for their use to efficiently convert biomass at yields sufficient for an industrial process. For commodity chemicals, yield from substrate is perhaps the most important economic factor. In an attempt to improve even further the ability of  C. bescii  to use xylan, we introduced two xylanases from  Acidothermus cellulolyticus . Acel_0180 includes tandem carbohydrate-binding modules (CBM2 and CBM3) located at the C-terminus, one of which, CBM2, is not present in  C. bescii . Also, the sequences of Xyn10A and Acel_0180 have very little homology with the GH10 domains present in  C. bescii . For these reasons, we selected these xylanases as potential candidates for synergistic interaction with those in the  C. bescii  exoproteome.    Results  Heterologous expression of two xylanases from  Acidothermus cellulolyticus  in  Caldicellulosiruptor bescii  resulted in a modest, but significant increase in the activity of the exoproteome of  C. bescii  on xylan substrates. Even though the increase in extracellular activity was modest, the ability of  C. bescii  to grow on these substrates was dramatically improved suggesting that the xylan substrate/microbe interaction substantially increased deconstruction over the secreted free enzymes alone.    Conclusions  We anticipate that the ability to efficiently use xylan, a major component of plant cell walls for conversion of plant biomass to products of interest, will allow the conversion of renewable, sustainable, and inexpensive plant feedstocks to products at high yields.    Electronic supplementary material  The online version of this article (doi:10.1186/s13068-016-0588-9) contains supplementary material, which is available to authorized users.

biotechnology for biofuels

Heterologous expression of family 10 xylanases from Acidothermus cellulolyticus enhances the exoproteome of Caldicellulosiruptor bescii and growth on xylan substrates