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Polysaccharides are structurally complex and essential constituents of life, and therefore, studies directed to these kinds of molecules have received scientific attention. Despite an easy availability of  Dolichos biflorus  Linn and  Trachyspermum ammi  (Linn) seeds isolation, characterization and antimicrobial studies of polysaccharides derived from these two natural sources have not been investigated. Therefore, we report here isolation of polysaccharides, their purification and characterization from  Dolichos biflorus  Linn and  Trachyspermum ammi  (Linn) seeds. Gel permeation chromatography, GC–MS, SEM, XRD, EDX and FT-IR analyses show the presence of three pentose sugar such as  d -ribose,  d -arabinose,  d -xylose and hexose sugar such as  d -mannose,  d -galactose and  d -glucose. Unprecedented antimicrobial activity of these polysaccharides against Gram positive bacteria such as  Staphylococcus aureus  and  Bacillus subtilis  and Gram negative bacteria such as  Escherichia coli  and  Pseudomonas aeruginosa  are established.

Polysaccharides from Dolichos biflorus Linn and Trachyspermum ammi Linn seeds: isolation, characterization and remarkable antimicrobial activity