English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The extraction of bioactive compounds from herbal materials requires optimization in order to recover the highest active dose. Response surface methodology was used to optimize variables affecting the microwave extraction of zerumbone from  Zingiber zerumbet  using the Box–Behnken design. The influence of variables, such as ethanol concentration (X 1 ), microwave power (X 2 ), irradiation time (X 3 ), and liquid-to-solid ratio (X 4 ), on the extraction of zerumbone was modeled using a second-order regression equation. The antiproliferative activity of optimized and non-optimized extracts was evaluated against the HeLa cancer cell line using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.    Results  Two linear parameters, X 1  and X 4 , and their quadratic parameters were highly significant at the P < 0.01 level. Two interaction parameters, X 1 X 4  and X 2 X 3  were significant, whereas interactions of X 1 X 2 , X 1 X 3 , X 2 X 4  and X 3 X 4  were insignificant (P > 0.05). The optimum microwave extraction conditions were as follows: ethanol concentration, 44%; microwave power, 518 W; irradiation time, 38.5 s; and liquid-to-solid ratio, 38 mL/g. Under these conditions, the maximum zerumbone yield was 5.88 mg/g DM, which was similar to the predicted value (5.946 mg/g DM). Optimized and non-optimized  Z. zerumbet  rhizome extracts exhibited significant antiproliferative activity against HeLa cancer cells, with half-maximal inhibitory concentration (IC 50 ) values of 4.3 and 7.8 μg/mL, respectively, compared with 1.68 μg/mL for the anticancer drug cisplatin. When the extract concentration increased from 4.3 to 16.0 μg/mL, the inhibition of cancer cell growth increased from 50.0 to 79.5%.    Conclusions  In this study, the optimized microwave protocol developed for extracting zerumbone from  Z. zerumbet  was faster and consumed less solvent than previous methods, while improving and enhancing the antiproliferative activity.

chemistry central journal

Optimization of microwave-assisted extraction of zerumbone from Zingiber zerumbet L. rhizome and evaluation of antiproliferative activity of optimized extracts