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Background  Progressive atrophic rhinitis (PAR) in pigs is caused by toxigenic  Pasteurella multocida . In Switzerland, PAR is monitored by selective culture of nasal swabs and subsequent polymerase chain reaction (PCR) screening of bacterial colonies for the  P.   multocida toxA  gene. A panel of 203 nasal swabs from a recent PAR outbreak were used to evaluate a novel quantitative real-time PCR for toxigenic  P.   multocida  in porcine nasal swabs.    Results  In comparison to the conventional PCR with a limit of detection of 100 genome equivalents per PCR reaction, the real-time PCR had a limit of detection of 10 genome equivalents. The real-time PCR detected  toxA -positive  P.   multocida  in 101 samples (49.8%), whereas the conventional PCR was less sensitive with 90  toxA -positive samples (44.3%). In comparison to the real-time PCR, 5.4% of the  toxA -positive samples revealed unevaluable results by conventional PCR.    Conclusions  The approach of culture-coupled  toxA  PCR for the monitoring of PAR in pigs is substantially improved by a novel quantitative real-time PCR.    Electronic supplementary material  The online version of this article (doi:10.1186/s13028-016-0267-7) contains supplementary material, which is available to authorized users.

A novel quantitative real-time polymerase chain reaction method for detecting toxigenic Pasteurella multocida in nasal swabs from swine