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Background  The total flavonoids from ethanol extract of the aerial part of  Ziziphora clinopodioides  Lam. (Lamlaceae) ( Xintahua ) showed protective activities against rat acute myocardial ischemia in rats. This study aims to isolate acacetin, a flavonoid, from the aerial part of  Z. clinopodioides , to develop an HPLC method for its detection, and to evaluate its protective effects on neonatal rat cardiomyocytes.    Methods  Sephadex LH-20 silicagel and pillar layer chromatography silica gel were applied for the isolation and purification of acacetin and its structure was elucidated on the basis of  1 H and  13 C NMR spectroscopy. The content of acacetin in  Z. clinopodioides  collected from three different origins was determined by HPLC. The neonatal rat cardiomyocytes were isolated and cultured  in vitro  to establish a hypoxia/reoxygenation injury model. The viability of cardiomyocytes was measured by the MTT method. Changes of malondialdehyde (MDA) content in the medium were also determined.    Results  The acacetin content in various batches of  Z. clinopodioides  ranged from 45.50 to 47.41 μg/g. Acacetin of 25, 10, 5 μg/mL significantly decreased the MDA content in a model of hypoxia/reoxygenation injury ( P  < 0.001,  P  < 0.001 and  P  = 0.033, respectively).    Conclusions  Acacetin protects neonatal cardiomyocytes from the damage induced by hypoxia/reoxygenation stress through reduction of lipid peroxidation and enhancement of the antioxidant activity.

Protective effects of acacetin isolated from Ziziphora clinopodioides Lam. (Xintahua) on neonatal rat cardiomyocytes