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Background Ralstonia solanacearum , one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabidopsis- R. solanacearum  system. However, the progress of  R. solanacearum  infection in Arabidopsis is still unclear. Results We generated a bioluminescent  R. solanacearum  by expressing plasmid-based  lux CDABE. Expression of  lux CDABE did not alter the bacterial growth and pathogenicity. The light intensity of bioluminescent  R. solanacearum  was linearly related to bacterial concentrations from 10 4  to 10 8  CFU·mL −1 . After root inoculation with bioluminescent  R. solanacearum  strain, light signals in tomato and Arabidopsis were found to be transported from roots to stems via the vasculature. Quantification of light intensity from the bioluminescent strain accurately reported the difference in disease resistance between Arabidopsis wild type and resistant mutants. Conclusions Bioluminescent  R. solanacearum  strain spatially and quantitatively measured bacterial growth in tomato and Arabidopsis, and offered a tool for the high-throughput study of  R. solanacearum -Arabidopsis interaction in the future.

Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology