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Background  Stalk lodging (breaking of plant stems prior to harvest) is a major impediment to increasing agricultural yields of grain crops. Rind puncture resistance is commonly used to predict the lodging resistance of several crop species. However, there exist no standard operating procedures or suggested protocols for conducting rind penetration experiments. In addition, experimental details of rind penetration tests such as the shape and size of the penetrating probe are rarely reported in the literature. This has prevented meta-analysis of results and has likewise prevented key findings of past studies from being replicated. As a first step towards establishing an agreed upon measurement standard for rind puncture resistance this study investigates the effect of the puncturing probe’s geometry on test results.    Results  Results demonstrate that probe geometry has a significant impact on test results. In particular, results showed that a 2 mm diameter chamfered probe produced stronger correlations with stalk bending strength than a 1.5 mm diameter pointed probe. The chamfered probe was also more strongly correlated with geometric features of the stalk that are known to influence stalk lodging resistance (e.g., rind thickness, diameter and section modulus). In addition, several alternative rind penetration metrics were investigated, and some were found to be superior to the most common rind penetration metric of maximum load.    Conclusions  There is a need in the agricultural and plant science community to create agreed-upon operating procedures and testing standards related to mechanical traits of plant stems. In particular, a standardized probe geometry and insertion rate for rind penetration studies are needed to enable greater interoperability and meta-analysis of results. Probe shape and size should be reported in any study conducting rind penetration tests as these factors significantly impact test results.

The effect of probe geometry on rind puncture resistance testing of maize stalks