English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  Past research has shown that virus-induced phytoene desaturase ( PDS ) gene silencing via agroinjection in the attached and detached fruit of tomato plants results in a pale-yellow fruit phenotype. Although the  PDS  gene is often used as a marker for gene silencing in tomatoes, little is known about the role of  PDS  in fruit ripening. In this study, we investigated whether the pepper  PDS  gene silenced endogenous  PDS  genes in the fruit of two tomato cultivars, Dotaerang Plus and Legend Summer.    Results  We found that the pepper  PDS  gene successfully silenced endogenous  PDS  in tomato fruit at a silencing frequency of 100% for both cultivars. A pale-yellow silenced area was observed over virtually the entire surface of individual fruit due to the transcriptional reduction in phytoene desaturase ( PDS ), zeta-carotene ( ZDS ), prolycopene isomerase ( CrtlSO ), and beta-carotene hydroxylase ( CrtR - b2 ), which are the carotenoid biosynthesis genes responsible for the red coloration in tomatoes.  PDS  silencing also affected the expression levels of the fruit-ripening genes Tomato AGAMOUS-LIKE1 ( TAGL1 ), RIPENING INHIBITOR ( RIN ), pectin esterase gene ( PE ), lipoxygenase ( LOX ), FRUITFULL1/FRUITFUL2 ( FUL1/FUL2 ), and the ethylene biosynthesis and response genes 1-aminocyclopropane-1-carboxylate oxidase 1 and 3 ( ACO1  and  ACO3 ) and ethylene-responsive genes ( E4  and  E8 ).    Conclusion  These results suggest that  PDS  is a positive regulator of ripening in tomato fruit, which must be considered when using it as a marker for virus-induced gene silencing (VIGS) experiments in order to avoid fruit-ripening side effects.

Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes