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Background   Solanum lycopersicum , an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots. However, a method to easily screen candidate genes which could play an important role during tomato mycorrhization is required.    Results  We have developed an optimized procedure for composite tomato plant obtaining achieved through  Agrobacterium rhizogenes -mediated transformation. This protocol involves the unusual in vitro culture of composite plants between two filter papers placed on the culture media. In addition, we show that  DsRed  is an appropriate molecular marker for the precise selection of cotransformed tomato hairy roots . S. lycopersicum  composite plant hairy roots appear to be colonized by the AM fungus  Rhizophagus irregularis  in a manner similar to that of normal roots, and a modified construct useful for localizing the expression of promoters putatively associated with mycorrhization was developed and tested.    Conclusions  In this study, we present an easy, fast and low-cost procedure to study AM symbiosis in tomato roots.

An improved method for Agrobacterium rhizogenes-mediated transformation of tomato suitable for the study of arbuscular mycorrhizal symbiosis