English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) in North America, Asia and Europe has caused severe economic loss to the global swine industry. However, the virome of PEDV infected pigs and its effect on disease severity remains unknown. The advancements of sequencing technology have made it possible to characterize the entire microbiome of different body sites for any host.    Methods  The objective of this study was to characterize the RNA virome in PEDV-positive pigs using the hypothesis-free metagenomics approach based on next-generation sequencing.  Specifically, 217 PEDV-positive swine fecal swab samples collected from diarrheic piglets over 17 US states during 2015–2016 were analyzed.    Results  A Kraken algorithm-based bioinformatics analysis revealed the presence of up to 9 different RNA genera besides PEDV ( Alphacoronavirus  genus), including  Mamastrovirus  (52%, 113/217),  Enterovirus  (39%, 85/217),  Sapelovirus  (31%, 67/217),  Posavirus  (30%, 66/217),  Kobuvirus  (23%, 49/217),  Sapovirus  (13%, 28/217),  Teschovirus  (10%, 22/217),  Pasivirus  (9%, 20/217), and  Deltacoronavirus  (3%, 6/217). There were 58 out of 217 piglets (27%) have PEDV infection alone whereas the remaining 159 (73%) shed 2 up to 9 different viruses.    Conclusion  These findings demonstrated that PEDV infected diarrheic pigs had an extensive RNA viral flora consisting of four different families:  Astroviridae, Picornaviridae, Caliciviridae , and  Coronaviridae .

virology journal

Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States