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Through a genome-wide screen we have identified calcium-tolerant deletion mutants for five genes in the budding yeast  Saccharomyces cerevisiae . In addition to  CNB1  and  RCN1  that are known to play a role in the calcium signalling pathway, the protein kinase gene  CMK2 , the sphingolipid homeostasis-related gene  ORM2  and the gene  SIF2  encoding the WD40 repeat-containing subunit of Set3C histone deacetylase complex are involved in the calcium sensitivity of yeast cells to extracellular calcium. Cmk2 and the transcription factor Crz1 have opposite functions in the response of yeast cells to calcium stress. Deletion of  CMK2  elevates the level of calcium/calcineurin signalling and increases the expression level of  PMR1  and  PMC1 , which is dependent on Crz1. Effects of Cmk2 on calcium sensitivity and calcium/calcineurin signalling are dependent on its kinase activity. Therefore, Cmk2 is a negative feedback controller of the calcium/calcineurin signalling pathway. Furthermore, the  cmk2 crz1  double deletion mutant is more resistant than the  crz1  deletion mutant, suggesting that Cmk2 has an additional Crz1-independent role in promoting calcium tolerance.   Electronic supplementary material  The online version of this article (10.1186/s12964-019-0320-z) contains supplementary material, which is available to authorized users.

cell communication and signaling

The protein kinase Cmk2 negatively regulates the calcium/calcineurin signalling pathway and expression of calcium pump genes PMR1 and PMC1 in budding yeast