English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder with various manifestations and complex etiology. Follicular fluid (FF) serves as the complex microenvironment for follicular development. However, the correlation between the concentration of steroid in FF and the pathogenesis of PCOS is still unclear.    Methods  Twenty steroid levels in FF from ten patients with PCOS and ten women with male-factor infertility undergoing in vitro fertilization were tested by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in order to explore their possibly correlation with PCOS. Meanwhile, the mRNA levels of core enzymes in steroid synthesis pathway from exosomes of FF were also detected by qPCR.    Results  The estriol ( p  < 0.01), estradiol ( p  < 0.05) and prenenolone ( p  < 0.01) levels in FF of PCOS group were significantly increased, compared to the normal group, and the progesterone levels ( p  < 0.05) were decreased in PCOS group. Increased mRNA levels of  CYP11A, CYP19A  and  HSD17B2  of exosomes were accompanied by the hormonal changes in FF. Correlation analysis showed that mRNA levels of  CYP11A  and  HSD17B2  were negatively correlated with percent of top-quality embryos and rate of embryos develop to blastocyst.    Conclusion  Our results suggest that increased levels of estrogen and pregnenolone in follicular fluid may affect follicle development in PCOS patients, and the mechanism is partially related to  HSD17B1 ,  CYP19A1  and  CYP11A1  expression change in FF exosomes.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12958-021-00749-6.

reproductive biology and endocrinology

Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome