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Background  Ovulation and luteinization of follicles are complex biological processes initiated by the preovulatory luteinizing hormone surge. The objective of this study was to identify genes that are differentially expressed in bovine granulosa cells (GC) of ovulatory follicles.    Methods  Granulosa cells were collected during the first follicular wave of the bovine estrous cycle from dominant follicles (DF) and from ovulatory follicles (OF) obtained 24 h following injection of human chorionic gonadotropin (hCG). A granulosa cell subtracted cDNA library (OF-DF) was generated using suppression subtractive hybridization and screened.    Results  Detection of genes known to be upregulated in bovine GC during ovulation, such as  ADAMTS1 ,  CAV1 ,  EGR1 ,  MMP1 ,  PLAT ,  PLA2G4A ,  PTGES ,  PTGS2 ,  RGS2 ,  TIMP1 ,  TNFAIP6  and  VNN2  validated the physiological model and analytical techniques used. For a subset of genes that were identified for the first time, gene expression profiles were further compared by semiquantitative RT-PCR in follicles obtained at different developmental stages. Results confirmed an induction or upregulation of the respective mRNAs in GC of OF 24 h after hCG-injection compared with those of DF for the following genes:  ADAMTS9 ,  ARAF ,  CAPN2 ,  CRISPLD2 ,  FKBP5 ,  GFPT2 ,  KIT ,  KITLG ,  L3MBLT3 ,  MRO ,  NUDT10 ,  NUDT11 ,  P4HA3 ,  POSTN ,  PSAP ,  RBP1 ,  SAT1 ,  SDC4 ,  TIMP2 ,  TNC  and  USP53 . In bovine GC,  CRISPLD2  and  POSTN  mRNA were found as full-length transcript whereas  L3MBLT3  mRNA was alternatively spliced resulting in a truncated protein missing the carboxy-terminal end amino acids,  774 KNSHNEL 780 . Conversely,  L3MBLT3  is expressed as a full-length mRNA in a bovine endometrial cell line. The  774 KNSHNEL 780  sequence is well conserved in all mammalian species and follows a SAM domain known to confer protein/protein interactions, which suggest a key function for these amino acids in the epigenetic control of gene expression.    Conclusions  We conclude that we have identified novel genes that are upregulated by hCG in bovine GC of OF, thereby providing novel insight into peri-ovulatory regulation of genes that contribute to ovulation and/or luteinization processes.    Electronic supplementary material  The online version of this article (10.1186/s12958-017-0306-x) contains supplementary material, which is available to authorized users.

Gene expression profiling of upregulated mRNAs in granulosa cells of bovine ovulatory follicles following stimulation with hCG