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Background  The purpose of this study was to test the hypothesis that estradiol (E 2 ) level on day 3 may be associated with  in vitro  fertilization (IVF) outcomes.    Methods  The records of patients who received clomiphene citrate 100 mg/day plus human menopausal gonadotropin 150 IU/day from day 3 and received frozen-thawed embryo transfers were reviewed. Patients were divided into three groups: group A (E 2  ≤30 pg/ml), group B (30< E 2  ≤50 pg/ml), and group C (E 2  >50 pg/ml). A total of 1080 cycles from 941 patients were included.    Results  The number of eggs and MII oocytes were less in group C than group A (both,  P  = 0.001). The embryo implantation ( P  = 0.006) and clinical pregnancy rates ( P  = 0.036) were lower in group C than group B, and the rates were similar between group A and B.    Conclusion  Maintaining the serum E 2  level from 30 to 50 pg/ml may result in a higher clinical pregnancy rate in IVF cycles.

reproductive biology and endocrinology

The association of serum estradiol level with outcomes of clomiphene citrate/human menopausal gonadotropin ovarian stimulation for in vitro fertilization and embryo transfer