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Background   Helicobacter pylori  ( H. pylori ) infection remains a global public health issue, especially in Asia. Due to the emergence of antibiotic-resistant strains and the complexity of  H. pylori  infection, conventional vaccination is the best way to control the disease. Our previous study found that the  N -acetyl-neuroaminyllactose-binding hemagglutinin protein (HpaA) is an effective protective antigen for vaccination against  H. pylori  infection, and intranasal immunization with the immunodominant HpaA epitope peptide (HpaA 154-171, P22, MEGVLIPAGFIKVTILEP) in conjunction with a CpG adjuvant decreased bacterial colonization in  H. pylori -infected mice. However, to confer more robust and effective protection against  H. pylori  infection, an optimized delivery system is needed to enhance the P22-specific memory T cell response.    Results  In this study, an intranasal nanoemulsion (NE) delivery system offering high vaccine efficacy without obvious cytotoxicity was designed and produced. We found that this highly stable system significantly prolonged the nasal residence time and enhanced the cellular uptake of the epitope peptide, which powerfully boosted the specific Th1 responses of the NE-P22 vaccine, thus reducing bacterial colonization without CpG. Furthermore, the protection efficacy was further enhanced by combining the NE-P22 vaccine with CpG.    Conclusion  This epitope-loaded nanoemulsion delivery system was shown to extend antigen release and elicit potent Th1 response, it is an applicable delivery system for intranasal vaccine against  H. pylori .Electronic supplementary material  The online version of this article (10.1186/s12951-019-0441-y) contains supplementary material, which is available to authorized users.

journal of nanobiotechnology

Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori