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Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by  ex cellulo  quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics.   Electronic supplementary material  The online version of this article (10.1186/s12951-018-0395-5) contains supplementary material, which is available to authorized users.

Protein fishing from single live cells