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Background  The mechanism of cholesteryl ester transfer protein (CETP) in lipid metabolism is still unclear. Furthermore, the relationship of CETP and atherosclerosis (AS) has been controversial. As pigs are a good model for both lipid and AS research, we investigated the lipid metabolism of human  CETP  ( hCETP ) transgenic pigs and explored the mechanism of CETP in lipid modulation.    Methods  Plasmids expressing the  hCETP  gene were designed, successfully constructed, and transfected into porcine fetal fibroblasts by liposomes. Using somatic cell nuclear transfer technology and embryonic transfer,  hCETP  transgenic pigs were generated. After the DNA, RNA, and protein levels were identified, positive  hCETP  transgenic pigs were selected. Blood samples were collected at different ages to evaluate the phenotypes of biochemical markers, and the metabolomes of plasma samples were analyzed by liquid mass spectrometry.    Results  Eight positive  hCETP  transgenic pigs and five negative cloned pigs were generated by transgenic technology. Finally, five  hCETP  transgenic and five cloned pigs were grown healthily. After feeding with a normal diet,  hCETP  transgenic pigs compared with unmodified pigs had no significant differences in body weight, liver function, kidney function, or plasma ions, while total cholesterol and low-density lipoprotein were higher than in unmodified pigs, and high-density lipoprotein was significantly decreased. Metabolomics analysis showed that there were differences in metabolic components between  hCETP  transgenic pigs, cloned pigs, and unmodified pigs.    Conclusions  In this study, we created  hCETP  transgenic pigs that could serve as an excellent model for lipid disorders and atherosclerosis.    Electronic supplementary material  The online version of this article (10.1186/s12944-017-0563-x) contains supplementary material, which is available to authorized users.

A novel swine model for evaluation of dyslipidemia and atherosclerosis induced by human CETP overexpression