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Background  Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck. LSCC patients have seriously impaired vocal, respiratory, and swallowing functions with poor prognosis. Circular RNA (circRNA) has attracted great attention in cancer research. However, the expression patterns and roles of circRNAs in LSCC remain largely unknown.    Methods  RNA sequencing was performed on 57 pairs of LSCC and matched adjacent normal mucosa tissues to construct circRNA, miRNA, and mRNA expression profiles. RT-PCR, qPCR, Sanger sequencing, and FISH were undertaken to study the expression, localization, and clinical significance of  circCORO1C  in LSCC tissues and cells. The functions of  circCORO1C  in LSCC were investigated by RNAi-mediated knockdown, proliferation analysis, EdU staining, colony formation assay, Transwell assay, and apoptosis analysis. The regulatory mechanisms among  circCORO1C ,  let-7c-5p , and  PBX3  were investigated by luciferase assay, RNA immunoprecipitation, western blotting, and immunohistochemistry.    Results   circCORO1C  was highly expressed in LSCC tissues and cells, and this high expression was closely associated with the malignant progression and poor prognosis of LSCC. Knockdown of  circCORO1C  inhibited the proliferation, migration, invasion, and in vivo tumorigenesis of LSCC cells. Mechanistic studies revealed that  circCORO1C  competitively bound to  let-7c-5p  and prevented it from decreasing the level of  PBX3 , which promoted the epithelial–mesenchymal transition and finally facilitated the malignant progression of LSCC.    Conclusions   circCORO1C  has an oncogenic role in LSCC progression and may serve as a novel target for LSCC therapy.  circCORO1C  expression has the potential to serve as a novel diagnostic and prognostic biomarker for LSCC detection.

Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis