English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background The carbapenem-resistance genes  bla  VIM  are widely disseminated in  Pseudomonas , and frequently harbored within class 1 integrons that reside within various mobile genetic elements (MGEs). However, there are few reports on detailed genetic dissection of  bla  VIM -carrying MGEs in  Pseudomonas . Methods This study presented the complete sequences of five  bla  VIM-2/-4 -carrying MGEs, including two plasmids, two chromosomal integrative and mobilizable elements (IMEs), and one chromosomal integrative and conjugative element (ICE) from five different  Pseudomonas  isolates. Results The two plasmids were assigned to a novel incompatibility (Inc) group Inc pSTY , which included only seven available plasmids with determined complete sequences and could be further divided into three subgroups Inc pSTY -1/2/3. A detailed sequence comparison was then applied to a collection of 15 MGEs belonging to four different groups: three representative Inc pSTY  plasmids, two Tn 6916 -related IMEs, two Tn 6918 -related IMEs, and eight Tn 6417 -related ICEs and ten of these 15 MGEs were first time identified. At least 22 genes involving resistance to seven different categories of antibiotics and heavy metals were identified within these 15 MGEs, and most of these resistance genes were located within the accessory modules integrated as exogenous DNA regions into these MGEs. Especially, eleven of these 15 MGEs carried the  bla  VIM  genes, which were located within 11 different concise class 1 integrons. Conclusion These  bla  VIM -carrying integrons were further integrated into the above plasmids, IMEs/ICEs with intercellular mobility. These MGEs could transfer between  Pseudomonas  isolates, which resulted in the accumulation and spread of  bla  VIM  among  Pseudomonas  and thus was helpful for the bacteria to survival from the stress of antibiotics. Data presented here provided a deeper insight into the genetic diversification and evolution of VIM-encoding MGEs in  Pseudomonas .

annals of clinical microbiology and antimicrobials

VIM-encoding IncpSTY plasmids and chromosome-borne integrative and mobilizable elements (IMEs) and integrative and conjugative elements (ICEs) in Pseudomonas