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Background  A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment.    Methods  We have designed and evaluated a bacterial biosensor expressing a luciferase reporter gene controlled by  pbr  and  cadA  promoters in  Cupriavidus metallidurans  (previously termed  Ralstonia metallidurans ) containing the  CH34  and  pI258  plasmids of  Staphylococcus aureus , respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated  pGL3-luc/pbr  biosensor and  pGL3-luc/cad  biosensor, that were based on the expression of luc+ and under the control of the  cad  promoter and the  cadC  gene of  S. aureus  plasmid  pI258  and  pbr  promoter and  pbrR  gene from plasmid  pMOL30  of  Cupriavidus metallidurans .    Results  We found that the  pGL3-luc/pbr  biosensor may be used to measure lead concentrations between 1–100 μM in the presence of other metals, including zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal. The  pGL3-luc/cad  biosensor could detect lead concentrations between 10 nM to 10 μM.    Conclusions  This biosensor was found to be specific for measuring lead ions in both environmental and biological samples.

biomedical engineering online

Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters