English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  While the World Health Organization (WHO) Southeast Asia region has the second highest incidence of malaria worldwide, malaria in Vietnam is focal to few provinces, where delayed parasite clearance to anti-malarial drugs is documented. This study aims to understand  Plasmodium  species distribution and the genetic diversity of  msp1  and  msp2  of parasite populations using molecular tools.    Methods  A total of 222 clinical isolates from individuals with uncomplicated malaria were subjected to  Plasmodium  species identification by nested real-time PCR. 166 isolates positive for  Plasmodium falciparum  mono infections were further genotyped for  msp1  (MAD20, K1, and RO33), and  msp2  allelic families (3D7 and FC27). Amplicons were resolved through capillary electrophoresis in the QIAxcel Advanced system.    Results  Mono-infections were high and with 75%  P. falciparum , 14%  Plasmodium vivax  and 9%  P. falciparum/P. vivax  co-infections, with less than 1%  Plasmodium malariae  identified. For  msp1 , MAD20 was the most prevalent (99%), followed by K1 (46%) allelic family, with no sample testing positive for RO33 (0%). For  msp2 , 3D7 allelic family was predominant (97%), followed by FC27 (10%). The multiplicity of infection of  msp1  and  msp2  was 2.6 and 1.1, respectively, and the mean overall multiplicity of infection was 3.7, with the total number of alleles ranging from 1 to 7.    Conclusions  Given the increasing importance of antimalarial drugs in the region, the genetic diversity of  P. falciparum msp1  and  msp2  should be regularly monitored with respect to treatment outcomes and/or efficacy studies in regions, where there are ongoing changes in the malaria epidemiology.

Molecular surveillance and temporal monitoring of malaria parasites in focal Vietnamese provinces