English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Establishing in vitro  Plasmodium falciparum  culture lines from patient parasite isolates can offer deeper understanding of geographic variations of drug sensitivity and mechanisms of malaria pathogenesis and immunity. Cellulose column filtration of blood is an inexpensive, rapid and effective method for the removal of host factors, such as leucocytes and platelets, significantly improving the purification of parasite DNA in a blood sample.    Methods  In this study, the effect of cellulose column filtration of venous blood on the initial in vitro growth of  P. falciparum  parasite isolates from Tanzanian children admitted to hospital was tested. The parasites were allowed to expand in culture without subcultivation until 5 days after admission or the appearance of dead parasites and parasitaemia was determined daily. To investigate whether the filtration had an effect on clonality,  P. falciparum  merozoite surface protein 2 genotyping was performed using nested PCR on extracted genomic DNA, and the  var  gene transcript levels were investigated, using quantitative PCR on extracted RNA, at admission and 4 days of culture.    Results  The cellulose-filtered parasites grew to higher parasitaemia faster than non-filtered parasites seemingly due to a higher development ratio of ring stage parasites progressing into the late stages. Cellulose filtration had no apparent effect on clonality or  var  gene expression; however, evident differences were observed after only 4 days of culture in both the number of clones and transcript levels of  var  genes compared to the time of admission.    Conclusions   Cellulose column filtration of parasitized blood is a cheap, applicable method for improving cultivation of  P. falciparum  field isolates for ex vivo based assays; however, when assessing phenotype and genotype of cultured parasites, in general, assumed to represent the in vivo infection, caution is advised.    Electronic supplementary material  The online version of this article (doi:10.1186/s12936-017-1714-2) contains supplementary material, which is available to authorized users.

Cellulose filtration of blood from malaria patients for improving ex vivo growth of Plasmodium falciparum parasites