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Background  Glutamate dehydrogenase of malaria parasites (pGDH) is widely used in rapid diagnostic tests for malaria. Variation in the  pGDH  gene among Korean isolates of  Plasmodium vivax  was analysed, and a recombinant pGDH protein was evaluated for use as antigens for the serodiagnosis of vivax malaria.    Methods  Genomic DNA was purified from blood samples of 20 patients and the  pGDH  gene of  P. vivax  was sequenced. Recombinant protein was prepared to determine the antigenicity of pGDH by enzyme-linked immunosorbent assay (ELISA).    Results  Partial sequence analysis of the  P. vivax pGDH  gene from the 20 Korean isolates showed that an open reading frame (ORF) of 1410 nucleotides encoded a deduced protein of 470 amino acids. The amino acid and nucleotide sequences were conserved among all the Korean isolates. This ORF showed 100% homology with  P. vivax  strain Sal-I (GenBank accession No. XP_001616617.1). The full ORF (amino acids 39–503), excluding the region before the intron, was cloned from isolate  P. vivax  Bucheon 3 (KJ726751) and subcloned into the expression vector pET28b for transformation into  Escherichia coli  BL21(DE3)pLysS. The expressed recombinant protein had a molecular mass of approximately 55 kDa and showed 84.8% sensitivity (39/46 cases) and 97.2% specificity (35/36 cases) in an ELISA. The efficacy of recombinant pGDH protein in seroepidemiological studies was also evaluated by ELISA using serum samples collected from 876 inhabitants of Gyodong-myeon, Ganghwa County, Incheon Metropolitan City. Of these samples, 91 (10.39%) showed a positive reaction with recombinant pGDH protein. Among the antibody-positive individuals, 13 (14.29%) had experienced malaria infection during the last 10 years.    Conclusion  The  pGDH  genes of  P. vivax  isolates from representative epidemic-prone areas of South Korea are highly conserved. Therefore, pGDH is expected to be a useful antigen in seroepidemiological studies. It was difficult to identify the foci of malaria transmission in Gyodong-myeon based on the patient distribution because of the very low parasitaemia of Korean vivax malaria. However, seroepidemiology with recombinant pGDH protein easily identified regions with the highest incidence of malaria within the study area. Therefore, recombinant pGDH protein may have a useful role in serodiagnosis.

Sequence conservation of Plasmodium vivax glutamate dehydrogenase among Korean isolates and its application in seroepidemiology