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Background  Strategies designed to advance towards malaria elimination rely on the detection and treatment of infections, rather than fever, and the interruption of malaria transmission between mosquitoes and humans. Mass drug administration with anti-malarials directed at eliminating parasites in blood, either to entire populations or targeting only those with malaria infections, are considered useful strategies to progress towards malaria elimination, but may be insufficient if applied on their own. These strategies assume a closer contact with populations, so incorporating a vector control intervention tool to those approaches could significantly enhance their efficacy. Ivermectin, an endectocide drug efficacious against a range of  Anopheles  species, could be added to other drug-based interventions. Interestingly, ivermectin could also be useful to target outdoor feeding and resting vectors, something not possible with current vector control tools, such as impregnated bed nets or indoor residual spraying (IRS).    Results   Anopheles aquasalis  susceptibility to ivermectin was assessed. In vivo assessments were performed in six volunteers, being three men and three women. The effect of ivermectin on reproductive fitness and mosquito survivorship using membrane feeding assay (MFA) and direct feeding assay (DFA) was assessed and compared. The ivermectin lethal concentration (LC) values were LC 50  = 47.03 ng/ml [44.68–49.40], LC 25  = 31.92 ng/ml [28.60–34.57] and LC 5  = 18.28 ng/ml [14.51–21.45]. Ivermectin significantly reduced the survivorship of  An. aquasalis  blood-fed 4 h post-ingestion ( X   2   [N = 880] = 328.16, p < 0.001), 2 days post-ingestion (DPI 2) ( X   2   [N = 983] = 156.75, p < 0.001), DPI 7 ( X   2   [N = 935] = 31.17, p < 0.001) and DPI 14 ( X   2   [N = 898] = 38.63, p < 0.001) compared to the blood fed on the untreated control. The average number of oviposited eggs per female was significantly lower in LC 5  group (22.44 [SD = 3.38]) than in control (34.70 [SD = 12.09]) ( X   2   [N = 199] = 10.52, p < 0.001) as well as the egg hatch rate (LC 5  = 74.76 [SD = 5.48]) (Control = 81.91 [SD = 5.92]) ( X   2   [N = 124] = 64.24, p < 0.001). However, no differences were observed on the number of pupae that developed from larvae (Control = 34.19 [SD = 10.42) and group (LC 5  = 33.33 [SD = 11.97]) ( X   2   [N = 124] = 0.96, p > 0.05).    Conclusions  Ivermectin drug reduces mosquito survivorship when blood fed on volunteer blood from 4 h to 14 days post-ingestion controlling for volunteers’ gender. Ivermectin at mosquito sub-lethal concentrations (LC 5 ) reduces fecundity and egg hatch rate but not the number of pupae that developed from larvae. DFA had significantly higher effects on mosquito survival compared to MFA. The findings are presented and discussed through the prism of malaria elimination in the Amazon region.

Filling gaps on ivermectin knowledge: effects on the survival and reproduction of Anopheles aquasalis, a Latin American malaria vector