English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   KLF5  is a member of the Kruppel-like factor, subfamily of zinc finger proteins that are involved in cancers.  KLF5  functions as a transcription factor and regulates the diverse protein-coding genes (PCGs) in colorectal cancer (CRC). However, the long non-coding RNAs (lncRNAs) regulated by  KLF5  in CRC are currently unknown.    Methods  In this study, we first designed a computational pipeline to determine the PCG and lncRNA targets of  KLF5  in CRC. Then we analyzed the motif pattern of the binding regions for the lncRNA targets. The regulatory co-factors of  KLF5  were then searched for through bioinformatics analysis. We also constructed a regulatory network for  KLF5  and annotated its functions. Finally, one of the  KLF5  lncRNA targets,  SNHG12 , was selected to further explore its expression pattern and functions in CRC.    Results  We were able to identify 19 lncRNA targets of  KLF5  and found that the motifs of the lncRNA binding sites were GC-enriched. Next, we pinpointed the transcription factors  AR  and  HSF1  as the regulatory co-factors of  KLF5  through bioinformatics analysis. Then, through the analysis of the regulatory network, we found that  KLF5  may be involved in DNA replication, DNA repair, and the cell cycle. Furthermore, in the cell cycle module, the  SNHG12  up-regulating expression pattern was verified in the CRC cell lines and tissues, associating it to CRC invasion and distal metastasis. This indicates that  SNHG12  may play a critical part in CRC tumorigenesis and progression. Additionally, expression of  SNHG12  was found to be down-regulated in CRC cell lines when  KLF5  expression was knocked-down by siRNA; and a strong correlation was observed between the expression levels of  SNHG12  and  KLF5 , further alluding to their regulatory relationship.    Conclusions  In conclusion, the network analysis of  KLF5  targets indicates that  SNHG12  may be a significant lncRNA in CRC.

Network analysis of KLF5 targets showing the potential oncogenic role of SNHG12 in colorectal cancer