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Background  Recent studies showed that long non-coding RNA (lncRNA) plays an important role in many life activities.  RPPH1  is one of the lncRNA genes that are expressed differently between breast cancer and normal tissues by the lncRNA gene chip. Our study was conducted to examine the regulation of lncRNA  RPPH1  in breast cancer.    Methods  Two cell lines, MCF-7 and MDA-MB-231, were selected to be the research objects in this study;  RPPH1  overexpression and knockdown models were established by transforming vectors. Real-time polymerase chain reaction, MTT assay, clone formation and cell flow cytometer assay were used to test the function of  RPPH1 . Dual-luciferase assay was used to detect a target relationship between  RPPH1  and miR-122.    Results    RPPH1  overexpression promoted cell cycle and proliferation and increased colony formation. In the  RPPH1  overexpression model, there was a target relationship between  RPPH1  and miR-122, and some of the downstream genes of miR-122, including  ADAM10, PKM2, NOD2  and  IGF1R , were increased. Moreover, we found that lentivirus-mediated interference of lncRNA  RPPH1  inhibited tumour growth in nude mice.    Conclusion  Breast cancer progression can be promoted by directly targeting miR-122 through lncRNA  RPPH1 . This study provided evidence that can serve as the molecular basis for improving treatment options for patients.

Inhibition of breast cancer cell proliferation and tumorigenesis by long non-coding RNA RPPH1 down-regulation of miR-122 expression