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Background  Cervical cancer is a major cause of death in women worldwide. Interferon-induced transmembrane protein 1 (IFITM1) is involved in antivirus defense, cell adhesion, and carcinogenesis in different tissues. However, the role of  IFITM1  gene in cervical squamous cell cancer is unclear.    Methods  To explore the role of IFITM1 in carcinogenesis of cervical cancer, we investigated the expression of  IFITM1  gene in cervical squamous cell carcinoma.  IFITM1  mRNA level was measured by real-time quantitative RT-PCR in cervical cancer tissues and their adjacent normal tissues. IFITM1 protein level was measured by immunohistochemistry. Methylation in the  IFITM1  gene promoter was detected by methylation-specific PCR. We then transfected HeLa cells with  IFITM1  expression vector or control vector. IFITM1 expression was examined; cell migration and invasion were analyzed by wound healing assay and matrigel-coated transwell migration assays, respectively. HeLa cell proliferation was measured by cell counting kit-8 assay and cell cycle analysis. Cell apoptosis was analyzed by Annexin V/propidium iodide double staining assay.    Results  The difference in IFITM1 protein expression between samples from chronic cervicitis and cervical carcinoma was statistically significant ( P  < 0.01). Ki-67 and PCNA protein expression levels were significantly higher in cervical cancer tissues than in their corresponding cervicitis tissues ( P  < 0.05 and  P  < 0.001, respectively).  IFITM1  mRNA level was significantly lower in cervical cancer tissues than in normal cervical tissues ( P  <  0.05 ). Methylation of the  IFITM1  gene promoter was significantly higher in cervical cancer than in normal cervical tissues ( P  < 0.05). Transfection of the  IFITM1  pcDNA3.1 construct decreased cell migration and invasion of HeLa cells, inhibited cell proliferation, and increased cell apoptosis.    Conclusion    IFITM1  gene expression may reduce the proliferation, migration, and invasion of cervical squamous cancer cells.

Down-regulation of IFITM1 and its growth inhibitory role in cervical squamous cell carcinoma