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Background  Aerobic growth provides benefits in biomass yield and stress tolerance of  Lactobacillus delbrueckii  subsp.  bulgaricus  ( L. bulgaricus ). Catabolite control protein A (CcpA) is a master regulator involved in the aerobic and anaerobic growth, metabolic production and stress response in  L. bulgaricus , but its potential molecular mechanisms remains unclear. The aim of this study is to elucidate the role of CcpA in  L. bulgaricus  in aerobic growth at the proteomic perspective.    Results  The differential proteomic analysis was performed on the  L. bulgaricus  ATCC11842 and its  ccpA  inactivated mutant strain using iTRAQ technology. A total of 132 differentially expressed proteins were obtained, among which 58 were up-regulated and 74 were down-regulated. These proteins were mainly involved in the cellular stress response, carbohydrate and energy metabolism, amino acid transport and protein synthesis, genetic information processing. Moreover, inactivation of  ccpA  negatively affected the expression of key enzymes involved in glycolysis pathway, while it enhanced the expression of proteins related to the pyruvate pathway, supporting the conclusion that CcpA mediated the shift from homolactic fermentation to mixed acid fermentation in  L. bulgaricus.Conclusions  Overall, these results showed that the role of CcpA in  L. bulgaricus  as a pleiotropic regulator in aerobic metabolism and stress response. This proteomic analysis also provide new insights into the CcpA-mediated regulatory network of  L. bulgaricus  and potential strategies to improve the production of starter and probiotic cultures based on the metabolic engineering of global regulators.

Effects of ccpA gene deficiency in Lactobacillus delbrueckii subsp. bulgaricus under aerobic conditions as assessed by proteomic analysis