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Background  Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cell-density mediated quorum sensing (QS). The disruption of QS, known as quorum quenching (QQ), is an effective and promising way to prevent and control pathogens, driving it be the potential bio-control agents. In our previous studies, AHL lactonase AiiK was identified with many characteristics, and constitutive expression vector pELX1 was constructed to express heterologous proteins in  Lactobacillus casei  MCJΔ1 ( L. casei  MCJΔ1). In this study, recombinant strain pELCW- aiiK / L. casei  MCJΔ1 ( Lc AiiK) and wild-type  Aeromonas hydrophila  ( A. hydrophila ) were co-cultured to test the QQ ability of  Lc AiiK against  A. hydrophila .    Results  A cell wall-associated expression vector pELCW for  L. casei  MCJΔ1 was constructed. Localization assays revealed that the expressed AiiK was anchored at the surface layer of  Lc AiiK via vector pELCW- aiiK .  Lc AiiK (OD 600  = 0.5) degraded 24.13 μM of C 6 -HSL at 2 h, 40.99 μM of C 6 -HSL at 12 h, and 46.63 μM of C 6 -HSL at 24 h. Over 50%  Lc AiiK cells maintained the pELCW- aiiK  plasmid after 15 generations of cultivation without erythromycin. Furthermore,  Lc AiiK inhibited the swimming motility, extracellular proteolytic activity, haemolytic activity and biofilm formation of  A. hydrophila  AH-1 and AH-4.    Conclusion  The AHL lactonase AiiK is firstly and constitutively expressed at the surface layer of  L. casei  MCJΔ1.  Lc AiiK displayed considerable AHL lactonase activity and great QQ abilities against  A. hydrophila  AH-1 and AH-4 by attenuating their QS processes instead of killing them. Therefore, the  Lc AiiK can be exploited as an anti-pathogenic drug or a bio-control agent to control the AHL-mediated QS of pathogenic bacteria.

microbial cell factories

Heterologous expression of AHL lactonase AiiK by Lactobacillus casei MCJΔ1 with great quorum quenching ability against Aeromonas hydrophila AH-1 and AH-4