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Background  The chemolithoautotrophic β-proteobacterium  Ralstonia eutropha  H16 ( Cupriavidus necator ) is one of the most studied model organisms for growth on H 2  and CO 2 .  R. eutropha  H16 is also a biologically significant bacterium capable of synthesizing O 2 -tolerant [NiFe]-hydrogenases (Hyds), which can be used as anode biocatalysts in enzyme fuel cells. For heterotrophic growth of  R. eutropha , various sources of organic carbon and energy can be used.    Results  Growth, bioenergetic properties, and oxidation–reduction potential (ORP) kinetics were investigated during cultivation of  R. eutropha  H16 on fructose and glycerol or lignocellulose-containing brewery spent grain hydrolysate (BSGH). BSGH was used as carbon and energy source by  R. eutropha  H16, and the activities of the membrane-bound hydrogenase (MBH) and cytoplasmic, soluble hydrogenase (SH) were measured in different growth phases. Growth of  R. eutropha  H16 on optimized BSGH medium yielded ~ 0.7 g cell dry weight L −1  with 3.50 ± 0.02 (SH) and 2.3 ± 0.03 (MBH) U (mg protein) −1  activities. Upon growth on fructose and glycerol, a pH drop from 7.0 to 6.7 and a concomitant decrease of ORP was observed. During growth on BSGH, in contrast, the pH and ORP stayed constant. The growth rate was slightly stimulated through addition of 1 mM K 3 [Fe(CN) 6 ], whereas temporarily reduced growth was observed upon addition of 3 mM dithiothreitol. The overall and  N , N ′-dicyclohexylcarbodiimide-sensitive ATPase activities of membrane vesicles were ~ 4- and ~ 2.5-fold lower, respectively, upon growth on fructose and glycerol (FGN) compared with only fructose utilization (FN). Compared to FN, ORP was lower upon bacterial growth on FGN, GFN, and BSGH.    Conclusions  Our results suggest that reductive conditions and low ATPase activity might be signals for energy depletion, which, in turn, leads to increased hydrogenase biosynthesis to overcome this unfavorable situation. Addition of fructose or microelements have no, or a negative, influence on hydrogenase activity. Organic wastes (glycerol, BSGH) are promising carbon and energy sources for the formation of biomass harboring significant amounts of the biotechnologically relevant hydrogenases MBH and SH. The results are valuable for using microbial cells as producers of hydrogenase enzymes as catalysts in enzymatic fuel cells.

microbial cell factories

Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic waste materials: growth characteristics, redox regulation and hydrogenase activity