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Background  Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO 2 . Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products.    Results  In this research, an electroactive succinate-producing cell factory was engineered in  E. coli  T110(pMtrABC, pFccA-CymA) by expressing  mtrABC, fccA  and  cymA  from  Shewanella oneidensis  MR-1, which can utilize electricity to reduce fumarate. The electroactive T110 strain was further improved by incorporating a carbon concentration mechanism (CCM). This strain was fermented in an MES system with neutral red as the electron carrier and supplemented with HCO 3  + , which produced a succinate yield of 1.10 mol/mol glucose—a 1.6-fold improvement over the parent strain T110.    Conclusions  The strain T110(pMtrABC, pFccA-CymA, pBTCA) is to our best knowledge the first electroactive microbial cell factory engineered to directly utilize electricity for the production of a specific product. Due to the versatility of the  E. coli  platform, this pioneering research opens the possibility of engineering various other cell factories to utilize electricity for bioproduction.    Electronic supplementary material  The online version of this article (10.1186/s12934-019-1067-3) contains supplementary material, which is available to authorized users.

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2