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Background  Low concentration NaHCO 3  (ca. 12 mM) had been demonstrated to be an excellent carbon source for industrially important green alga  Chlorella vulgaris  and high concentration NaHCO 3  (e.g. 160 mM) had been shown to be capable of controlling protozoa and stimulating lipid accumulation of another green alga, i.e.,  Neochloris oleoabundans . Furthermore, little was known about the mechanisms of the effects of NaHCO 3  on microalgae. Thorough studies on the effects of high NaHCO 3  on  C. vulgaris  and their mechanisms were therefore warranted.    Methods  We systematically compared the cell growth, lipid production, and cell morphology of the industrially important  C. vulgaris  in 160 mM NaHCO 3  or 160 mM NaCl media at different pH levels. These data allowed us to analyze the effects of total dissolved inorganic carbon (DIC) and individual DIC species on  C. vulgaris . Cell growth of  C. vulgaris  at a range of concentrations at 160 mM or lower was also studied.    Results  Cellular lipid cell content of 494 mg g −1  and lipid productivity of 44.5 mg L −1  day −1  were obtained at 160 mM NaHCO 3  and pH 9.5. High concentration NaHCO 3  (e.g. 160 mM) was inhibitive to cell growth but stimulating to lipid accumulation and caused unicellular  C. vulgaris  to transfer to colonial cells. Increasing pH in the range of 7.5–9.5 caused increasing inhibition to cell growth in 160 mM NaCl. Whereas the optimal pH for cell growth was 8.5 for 160 mM NaHCO 3  cultures. Comparative experiments with 0–160 mM NaHCO 3  indicate that 10 mM was the optimal concentration and increasing NaHCO 3  from 10 to 160 mM caused increasing inhibition to cell growth.    Conclusions  High concentration DIC was inhibitor to cell growth but stimulator to lipid accumulation of  C. vulgaris . It caused unicellular  C. vulgaris  to transform to colonial cells. Results suggest that high concentration of a particular DIC species, i.e., dCO 2 , was the primary stress responsible for cell growth inhibition. Where CO 3  2−  was likely the DIC species responsible for lipid stimulation of  C. vulgaris . Furthermore, we propose that the colony formation at high DIC conditions was employed by  C. vulgaris  to mitigate the stress by minimizing cell exposure to unfavorable environment.

Effects of sodium bicarbonate on cell growth, lipid accumulation, and morphology of Chlorella vulgaris