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Background  2′-Fucosyllactose (2-FL), one of the most abundant oligosaccharides in human milk, has potential applications in foods due to its health benefits such as the selective promotion of bifidobacterial growth and the inhibition of pathogenic microbial binding to the human gut. Owing to the limited amounts of 2-FL in human milk, alternative microbial production of 2-FL is considered promising. To date, microbial production of 2-FL has been studied mostly in  Escherichia coli . In this study, 2-FL was produced alternatively by using a yeast  Saccharomyces cerevisiae , which may have advantages over  E. coli .    Results  Fucose and lactose were used as the substrates for the  salvage  pathway which was constructed with  fkp  coding for a bifunctional enzyme exhibiting  l -fucokinase and guanosine 5′-diphosphate- l -fucose phosphorylase activities,  fucT2  coding for α-1,2-fucosyltransferase, and  LAC12  coding for lactose permease. Production of 2-FL by the resulting engineered yeast was verified by mass spectrometry. 2-FL titers of 92 and 503 mg/L were achieved from 48-h batch fermentation and 120-h fed-batch fermentation fed with ethanol as a carbon source, respectively.    Conclusions  This is the first report on 2-FL production by using yeast  S. cerevisiae . These results suggest that  S. cerevisiae  can be considered as a host engineered for producing 2-FL via the  salvage  pathway.    Electronic supplementary material  The online version of this article (10.1186/s12934-018-0947-2) contains supplementary material, which is available to authorized users.

Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiae