English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Lignocellulosic ethanol could offer a sustainable source to meet the increasing worldwide demand for fuel. However, efficient and simultaneous metabolism of all types of sugars in lignocellulosic hydrolysates by ethanol-producing strains is still a challenge.    Results  An engineered strain  Escherichia coli  B0013-2021HPA with regulated glucose utilization, which could use all monosaccharides in lignocellulosic hydrolysates except glucose for cell growth and glucose for ethanol production, was constructed. In  E. coli  B0013-2021HPA,  pta - ackA ,  ldhA  and  pflB  were deleted to block the formation of acetate, lactate and formate and additional three mutations at  glk ,  ptsG  and  manZ  generated to block the glucose uptake and catabolism, followed by the replacement of the wild-type  frdA  locus with the  ptsG  expression cassette under the control of the temperature-inducible λ pR and pL promoters, and the final introduction of pEtac-PA carrying  Zymomonas mobilis pdc  and  adhB  for the ethanol pathway. B0013-2021HPA was able to utilize almost all xylose, galactose and arabinose but not glucose for cell propagation at 34 °C and converted all sugars to ethanol at 42 °C under oxygen-limited fermentation conditions.    Conclusions  Engineered  E. coli  strain with regulated glucose utilization showed efficient metabolism of mixed sugars in lignocellulosic hydrolysates and thus higher productivity of ethanol production.    Electronic supplementary material  The online version of this article (10.1186/s12934-018-0915-x) contains supplementary material, which is available to authorized users.

Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization